Publications of the Department of Cellphysiology and Biophysics

Peer-reviewed journals

  • Becker A, Lehrich T, Kalies S, Heisterkamp A, Ngezahayo A (2019) Parameters for Optoperforation-Induced Killing of Cancer Cells Using Gold Nanoparticles Functionalized With the C-terminal Fragment of Clostridium Perfringens EnterotoxinInt J Mol Sci. 2019 Aug 30;20(17). pii: E4248
    DOI: 10.3390/ijms20174248
  • Berkelmann L, Bader A, Meshksar S, Dierks A, Hatipoglu Majernik G, Krauss JK, Schwabe K, Manteuffel D, Ngezahayo A (2019) Tumour-treating fields (TTFields): Investigations on the mechanism of action by electromagnetic exposure of cells in telophase/cytokinesisSci Rep. 2019 May 14;9(1):7362
    DOI: 10.1038/s41598-019-43621-9
  • Dierks A, Bader A, Lehrich T, Ngezahayo A (2019) Stimulation of the A2B Adenosine Receptor Subtype Enhances Connexin26 Hemichannel Activity in Small Airway Epithelial CellsCell Physiol Biochem 2019;53:606-622
    DOI: 10.33594/000000160
  • Schadzek P, Stahl Y, Preller M, Ngezahayo A (2019) Analysis of the dominant mutation N188T of human connexin46 (hCx46) using concatenation and molecular dynamics simulationFEBS Open Bio (in press)
    DOI: 10.1002/2211-5463.12624
  • Becker A, Leskau M, Schlingmann-Molina B, Hohmeier S, Alnajjar S, Murua Escobar H, Ngezahayo A (2018) Functionalization of gold-nanoparticles by the Clostridium perfringens enterotoxin C-terminus for tumor cell ablation using the gold nanoparticle-mediated laser perforation techniqueScient Rep (2018)8:14963
    DOI: 10.1038/s41598-018-33392-0
  • Schadzek P, Hermes D, Stahl Y, Dilger N, Ngezahayo A (2018) Concatenation of human connexin26 (hCx26) and human connexin46 (hCx46) for the analysis of heteromeric gap junction hemichannels and heterotypic gap junction channelsInt J Mol Sci 19(9)2742
    DOI: 10.3390/ijms19092742
  • Bader A, Bintig W, Begandt D, Klett A, Siller IG, Gregor C, Schaarschmidt F, Weksler B, Romero I, Couraud PO, Hell SW, Ngezahayo A (2017) Adenosine receptors regulate gap junction coupling of the human cerebral microvascular endothelial cells hCMEC/D3 by Ca2+ influx through CNG channelsJ Physiol 595:2497-2517
    DOI: 10.1113/JP273150
  • Berthoud VM, Ngezahayo A (2017) Focus on lens connexinsBMC Cell Biol;18(1):6
    DOI: 10.1186/s12860-016-0116-6
  • Harting TP, Stubbendorff M, Hammer SC, Schadzek P, Ngezahayo A, Murua Escobar H, Nolte I (2017) Dichloroacetate affects proliferation but not apoptosis in canine mammary cell linesPLoS One June 7
    DOI: 10.1371/journal.pone.0178744
  • Blödow A, Begandt D, Bader A, Becker A, Burghard A, Kühne D, Kral A, Ngezahayo A (2016) ATP-sensitive K+ channels (Kir6.1/SUR1) regulate gap junctional coupling in cochlear-supporting cellsPflugers Arch 468(7):1215-1222
    DOI: 10.1007/s00424-016-1815-8
  • Hammer SC, Becker A, Rateitschak K, Mohr A, Lüder Ripoli F, Hennecke S, Junginger J, Hewicker-Trautwein M, Brenig B, Ngezahayo A, Nolte I, Murua Escobar H (2016) Longitudinal Claudin Gene Expression Analyses in Canine Mammary Tissues and Thereof Derived Primary Cultures and Cell LinesInt J Mol Sci 17(10)1655
    DOI: 10.3390/ijms17101655
  • Hammer SC, Nagel S, Junginger J, Hewicker-Trautwein M, Wagner S, Heisterkamp A, Ngezahayo A, Nolte I, Murua Escobar H (2016) Claudin-1, -3, -4 and -7 gene expression analyses in canine prostate carcinoma and mammary tissue derived cell linesNeoplasma 63(2):231-238
    DOI: 10.4149/208_150924N505
  • Harting T, Stubbendorff M, Willenbrock S, Wagner S, Schadzek P, Ngezahayo A, Escobar HM, Nolte I (2016) The effect of dichloroacetate in canine prostate adenocarcinomas and transitional cell carcinomas in vitroInt J Oncol 49:2341-2350
    DOI: 10.3892/ijo.2016.3720
  • Kniggendorf AK, Nogueira R, Kelb C, Schadzek P, Meinhardt-Wollweber M, Ngezahayo A, Roth B (2016) Confocal Raman microscopy and fluorescent in situ hybridization - A complementary approach for biofilm analysisChemosph 161:112-118
    DOI: 10.1016/j.chemosphere.2016.06.096
  • Perin PM, Haid S, Brown RJ, Doerrbecker J, Schulze K, Zeilinger C, von Schaewen M, Heller B, Vercauteren K, Luxenburger E, Baktash YM, Vondran FW, Speerstra S, Awadh A, Mukhtarov F, Schang LM, Kirschning A, Müller R, Guzman CA, Kaderali L, Randall G, Meuleman P, Ploss A, Pietschmann T (2016) Flunarizine prevents hepatitis C virus membrane fusion in a genotype-dependent manner by targeting the potential fusion peptide within E1Hepatology 63(1)49-62
    DOI: 10.1002/hep.28111
  • Rapanas A, Bader A, Klett A, Ngezahayo A, Glasmacher B (2016) The effect of dipyridamole embedded in a drug delivery system made by electrospun nanofibers on aortic endothelial cellsJ Drug Deliv Sci Tech 35:343-352
    DOI: 10.1016/j.jddst.2016.08.011
  • Schadzek P, Schlingmann B, Schaarschmidt F, Lindner J, Koval M, Heisterkamp A, Ngezahayo A, Preller M (2016) Data of the molecular dynamics simulations of mutations in the human connexin46 docking interfaceData Brief 7:93-99
    DOI: 10.1016/j.dib.2016.01.067
  • Schadzek P, Schlingmann B, Schaarschmidt F, Lindner J, Koval M, Heisterkamp A, Preller M, Ngezahayo A (2016) The cataract related mutation N188T in human connexin46 (hCx46) revealed a critical role for residue N188 in the docking process of gap junction channelsBiochimica et Biophysica Acta - Biomembranes 1858 (1):57-66
    DOI: 10.1016/j.bbamem.2015.10.001
  • Begandt D, Bader A, Antonopoulos GC, Schomaker M, Kalies S, Meyer H, Ripken T, Ngezahayo A (2015) Gold nanoparticle-mediated (GNOME) laser perforation: a new method for a high-throughput analysis of gap junction intercellular couplingJ Bioenerg Biomembr 47:441-449
    DOI: 10.1007/s10863-015-9623-y
  • Hermane J, Bułyszko I, Eichner S, Sasse F, Collisi W, Poso A, Schax E, Walter JG, Scheper T, Kock K, Herrmann C, Aliuos P, Reuter G, Zeilinger C, Kirschning A (2015) New, non-quinone fluorogeldanamycin derivatives strongly inhibit Hsp90Chembiochem 16(2)302-311
    DOI: 10.1002/cbic.201402375
  • Schomaker M, Killian D, Willenbrock S, Heinemann D, Kalies S, Ngezahayo A, Nolte I, Ripken T, Junghanß C, Meyer H, Murua Escobar H, Heisterkamp A (2015) Biophysical effects in off-resonant gold nanoparticle mediated (GNOME) laser transfection of cell lines, primary- and stem cells using fs laser pulsesJ Biophotonics 8(8):646-658
    DOI: 10.1002/jbio.201400065
  • Kniggendorf AK, Meinhardt-Wollweber M, Yuan X, Roth B, Seifert A, Fertig N, Zeilinger C (2014) Temperature-sensitive gating of hCx26: high-resolution Raman spectroscopy sheds light on conformational changesBiomed Opt Express 5(7)2054-2065
    DOI: 10.1364/BOE.5.002054
  • Meyer W, Oberthuer A, Ngezahayo A, Neumann U, Jacob R (2014) Immunohistochemical demonstration of connexins in the developing feather follicle of the chickenActa Histochem 116(4):639-645
    DOI: 10.1016/j.acthis.2013.11.016
  • Schax E, Walter JG, Märzhäuser H, Stahl F, Scheper T, Agard DA, Eichner S, Kirschning A, Zeilinger C (2014) Microarray-based screening of heat shock protein inhibitorsJ Biotechnol 180:1-9
    DOI: 10.1016/j.jbiotec.2014.03.006
  • Wagner S, Ngezahayo A, Murua Escobar H, Nolte I (2014) Role of miRNA let-7 and its major targets in prostate cancerBiomed Res Int 2014:376326
    DOI: 10.1155/2014/376326
  • Begandt D, Bader A, Dreyer L, Eisert N, Reeck T, Ngezahayo A (2013) Biphasic increase of gap junction coupling induced by dipyridamole in the rat aortic A-10 vascular smooth muscle cell lineJ Cell Comm Signal 7:151-160
    DOI: 10.1007/s12079-013-0196-4
  • Begandt D, Bader A, Gerhard L, Lindner J, Dreyer L, Sclingmann B, Ngezahayo A (2013) Dipyridamole-related enhancement of gap junction coupling in the GM-7373 aortic endothelial cells correlates with an increase in the amount of connexin 43 mRNA and protein as well as gap junction plaquesJ Bioenerg Biomembr 45:409-419
    DOI: 10.1007/s10863-013-9518-8
  • Moulay M, Liu W, Willenbrock S, Sterenczak KA, Carlson R, Ngezahayo A, Murua Escobar H, Nolte I (2013) Evaluation of stem cell marker gene expression in canine prostate carcinoma- and prostate cyst-derived cell linesAnticancer Res 33(12):5421-5431
  • Schlie-Wolter S, Ngezahayo A, Chichkov BN (2013) The selective role of ECM components on cell adhesion, morphology, proliferation and communication in vitroExp Cell Res 319:1553-1561
    DOI: 10.1016/j.yexcr.2013.03.016
  • Schlingmann B, Schadzek P, Hemmerling F, Schaarschmidt F, Heisterkamp A, Ngezahayo A (2013) The role of the C-terminus in functional expression and internalization of rat connexin46 (rCx46)J Bioenerg Biomembr 45:59-70
    DOI: 10.1007/s10863-012-9480-x
  • Bintig W, Begandt D, Schlingmann B, Gerhard L, Pangalos M, Dreyer L, Hohnjec N, Couraud PO, Romero IA, Weksler BB, Ngezahayo A (2012) Purine receptors and Ca2+ signalling in the human blood–brain barrier endothelial cell line hCMEC/D3Purinergic Signal 8(1):71–80
    DOI: 10.1007/s11302-011-9262-7
  • Schlingmann B, Schadzek P, Busko S, Heisterkamp A, Ngezahayo A (2012) Cataract-associated D3Y mutation of human connexin46 (hCx46) increases the dye coupling of gap junction channels and suppresses the voltage sensitivity of hemichannelsJ Bioenerg Biomembr 44:607-614
    DOI: 10.1007/s10863-012-9461-0
  • Dreyer L, Krolitzki B, Autschbach R, Vogt P, Welte T, Ngezahayo A, Glasmacher B (2011) An Advanced Cone-And-Plate Reactor for the in vitro-Application of Shear Stress on Adherent CellsClin Hemorheol Microcirc 49(1-4)391-397
    DOI: 10.3233/CH-2011-1488
  • Pangalos M, Bintig W, Schlingmann B, Feyerabend F, Witte F, Begandt D, Heisterkamp A, Ngezahayo A (2011) Action potentials in primary osteoblasts and in the MG-63 osteoblast-like cell lineJ Bioenerg Biomembr 43(3)311–322
    DOI: 10.1007/s10863-011-9354-7
  • Baumgart J, Bintig W, Ngezahayo A, Lubatschowski H, Heisterkamp A (2010) Fs-laser-induced Ca2+ concentration change during membrane perforation for cell transfectionOpt Express 18:2219-2229
    DOI: 10.1364/OE.18.002219
  • Begandt D, Bintig W, Oberheide K, Schlie S, Ngezahayo A (2010) Dipyridamole increases gap junction coupling in bovine GM-7373 aortic endothelial cells by a cAMP-protein kinase A dependent pathwayJ Bioenerg Biomembr 42(1)79-84
    DOI: 10.1007/s10863-009-9262-2
  • Schlie S, Fadeeva E, Koch J, Ngezahayo A, Chichkov BN (2010) Femtosecond laser fabricated spike structures for selective control of cellular behaviorJ Biomat App 25(3)217-233
    DOI: 10.1177/0885328209345553
  • Schomaker M, Killian D, Willenbrock S, Diebold E, Mazur E, Bintig W, Ngezahayo A, Nolte I, Escobar HM, Junghanß C, Lubatschowski H, Heisterkamp A (2010) Ultrashort laser pulse cell manipulation using nano- and micro- materialsProc SPIE 7762
    DOI: 10.1117/12.869054
  • Baumgart J, Kuetemeyer K, Bintig W, Ngezahayo A, Ertmer W, Lubatschowski H, Heisterkamp A (2009) Repetition rate dependency of reactive oxygen species formation during femtosecond laser-based cell surgeryJ Biomed Opt 14(5):054040
    DOI: 10.1117/1.3253382
  • Bintig W, Baumgart J, Walter WJ, Heisterkamp A, Lubatschowski H, Ngezahayo A (2009) Purinergic signalling in rat GFSHR-17 granulosa cells: an in vitro model of granulosa cells in maturing folliclesJ Bioenerg Biomembr 41:85-94
    DOI: 10.1007/s10863-009-9199-5
  • Baumgart J, Bintig W, Ngezahayo A, Willenbrock S, Murua Escobar H, Ertmer W, Lubatschowski H, Heisterkamp A (2008) Quantified femtosecond laser based opto-perforation of living GFSHR-17 and MTH53 a cellsOpt Expr 16:3021-3031
    DOI: 10.1364/OE.16.003021
  • Fadeeva E, Schlie S, Koch J, Ngezahayo A, Chichkov BN (2008) The hydrophobic properties of femtosecond laser fabricated spike structures and their effects on cell proliferationPhysica Status Solidi
    DOI: 10.1002/pssa.200881063
  • Steffens M, Göpel F, Ngezahayo A, Zeilinger C, Ernst A, Kolb H-A (2008) Regulation of connexons composed of human connexin26 (hCx26) by temperatureBiochem Biophys Acta 1778:1206-1212
    DOI: 10.1016/j.bbamem.2008.01.016
  • Walter WJ, Zeilinger C, Bintig W, Kolb H-A, Ngezahayo A (2008) Phosphorylation in the C-terminus of the rat connexin46 (rCx46) and regulation of the conducting activity of the formed connexonsJ Bioenerg Biomembr 40:397-405
    DOI: 10.1007/s10863-008-9151-0
  • Heisterkamp A, Baumgart J, Maxwell IZ, Ngezahayo A, E Mazur E, Lubatschowski H (2007) Fs-laser scissors for photobleaching, ablation in fixed samples and living cells, and studies of cell mechanicsMeth Cell Biol 82,293-307
    DOI: 10.1016/S0091-679X(06)82009-1
  • Ovsianikov A, Schlie S, Ngezahayo A, Haverich A, Chichkov BN (2007) Two-photon polymerization technique for microfabrication of CAD-designed 3D scaffolds from commercially available photosensitive materialsJ Tissue Eng Regen Med 1(6):443-449
    DOI: 10.1002/term.57
  • Pätzold R, Keuntje M, Theophile K, Müller J, Mielcarek E, Ngezahayo A, Anders-von Ahlften A (2007) In situ mapping of nitrifiers and anammox bacteria in microbial aggregates by means of confocal resonance Raman microscopyJ Microb Meth 72(3)241-248
    DOI: 10.1016/j.mimet.2007.12.003
  • Schlie S, Ngezahayo A, Ovsianikov A, Fabian T, Kolb H-A, Haferkamp H, Chichkov BN (2007) Three-dimensional cell growth on structures fabricated from ORMOCER® by two-photon polymerisation techniqueJ Biomater Applic 22(3)275-287
    DOI: 10.1177/0885328207077590
  • Baumgart J, Heisterkamp A, Ngezahayo A, Ertmer W, Lubatschowski H (2006) Cell surgery by fs-laser pulsesProc SPIE, Commercial and Biomedical Applications of Ultrafast Lasers VII, 6108:271-279
  • Kikuchi T, Zhao HB, Ngezahayo A, White TW (2006) Gap Junctions and Cochlear HomeostasisJ Membr Biol 209:177-186
    DOI: 10.1007/s00232-005-0832-x
  • Ngezahayo A, Altmann B, Steffens M, Kolb H-A (2005) Gap Junction Coupling and Apoptosis in GFSHR-17 Granulosa CellsJ Membr Biol 204:137-144
    DOI: 10.1007/s00232-005-0756-5
  • Breidert S, Jakob R, Ngezahayo A, Kolb H-A, Naim HY (2004) Trafficking pathways of Cx49-GFP in living mammalian cellsBiol Chem 386:155-160
    DOI: 10.1515/BC.2005.019
  • Debarry M, Marten I, Ngezahayo A, Kolb H-A (2004) Differential defense responses in sweet potato suspension culturePlant Sci. 168:1171-1179
    DOI: 10.1016/j.plantsci.2004.12.016
  • Enders O, Ngezahayo A, Wiechmann M, Leisten F, Kolb H-A (2004) Structural calorimetry of main transition of supported DMPC bilayers by temperature controlled AFMBiophys J 87:2522-2531
    DOI: 10.1529/biophysj.104.040105
  • Blödow A, Ngezahayo A, Ernst A, Kolb H-A (2003) Calmodulin antagonists suppress gap junction coupling in isolated Hensen cells of the guinea pig cochleaPflügers Arch 446:36-41
    DOI: 10.1007/s00424-002-1004-9
  • Ngezahayo A, Altmann B, Kolb H-A (2003) Regulation of gap junctional coupling, ion fluxes and cell volume by cGMP in GFSHR-17 granulosa cellsJ Membr Biol 194:165-176
    DOI: 10.1007/s00232-003-2033-9
  • Todt I, Ngezahayo A, Ernst A, Kolb H-A (2001) Hydrogen peroxide inhibits gap junctional coupling and modulates intracellular free calcium in cochlear Hensen cellsJ Membr Biol 181:107-114
    DOI: 10.1007/s00232001-0014-4
  • Jedamzik B, Marten I, Ngezahayo A, Ernst A, Kolb H-A (2000) Regulation of Lens rCx46-formed Hemichannels by Activation of Protein Kinase C, External Ca2+ and ProtonsJ Membr Biol 173:39-46
    DOI: 10.1007/s002320001005
  • Ngezahayo A, Schachner M, Artola A (2000) Synaptic activity modulates the induction of bidirectional synaptic changes in adult mouse hippocampusJ Neurosci 20:2451-2458
    DOI: 10.1523/JNEUROSCI.20-07-02451.2000
  • Todt I, Ngezahayo A, Ernst A, Kolb H-A (1999) Inhibition of gap junctional coupling in cochlear supporting cells by GentamicinPflügers Arch 438:865-867
    DOI: 10.1007/s004249900109
  • Ngezahayo A, Zeilinger C, Todt I, Marten I, Kolb H-A (1998) Inactivation of expressed and conducting rCx46 hemichannels by phosphorylationPflügers Arch 436:627-629
    DOI: 10.1007/s004240050681
  • Ngezahayo A, Lang F, Kolb H-A (1995) Cholecystokinin-octapeptide affects the fluorescence signal of a single pancreatic acinar cell loaded with the acrylodan-labelled MARCKS peptide, a protein kinase C substratePflügers Arch 429:805-808
    DOI: 10.1007/BF00374804
  • Hartmann B, Ngezahayo A, Heller B, Jalowy A, Burkart V, Kolb H-A (1994) Nitric oxide toxicity in pancreatic islet cells: role of protein biosynthesis, calcium influx and arachidonic acid metabolismBiochem Soc Trans 22:23-26
    DOI: 10.1042/bst0220023
  • Ngezahayo A, Kolb H-A (1994) Regulation of gap junctional coupling in isolated pancreatic acinar cell pairs by cholecystokinin-octapeptide, vasoactive intestinal peptide (VIP) and a VIP-antagonistJ Membr Biol 139:127-136
    DOI: 10.1007/BF00232431
  • Ngezahayo A, Kolb H-A (1993) Gap junctional conductance tunes phase difference of cholecystokinin evoked calcium oscillations in pairs of pancreatic acinar cellsPflügers Arch 422:413-415
    DOI: 10.1007/BF00374302
  • Ngezahayo A, Kolb H-A (1990) Gap junctional permeability is affected by cell volume changes and modulates volume regulationFEBS Lett 276:6-8
    DOI: 10.1016/0014-5793(90)80493-3


  • Fadeeva E, Schlie S, Koch J, Vorobyev AY, Guo C, Chichkov BN (2009) Femtosecond laser-induced surface structures on platinum and their effects on hydrophobicity and fibroblast cell proliferationTextbook